CUHK-HKEH-PWH International Ophthalmology Fellowship
Basic Sciences Programme Director
Prof THAM Chee Yung Clement
S.H. Ho Professor of OVS
Honorary Chief of Service, HKEH
Director, CUHK Eye Centre
Deputy Director, Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong
Secretary General & CEO, Asia-Pacific Academy of Ophthalmology (APAO)
Prof LEUNG Kai Shun Christopher
Head, Graduate Division of Ophthalmology and Visual Sciences
We are committed to research of eye diseases at the highest standard and offer unique opportunities in the study of eye diseases using state-of-the-art research technologies in our laboratories. Our basic science research involves studies on the etiology, molecular genetics, cell biology, histopathology, biochemistry, and therapeutic responses of various eye diseases. We have established a comprehensive research laboratory system with modern facilities:
- Molecular Genetics and Genome Laboratories: automated sequencers, PCR and real-time PCR machines, DNA microarray scanner.
- Cell Biology Laboratories: laminar-flow clean bench, phase-contrast and fluorescence microscopes, ChemiDoc system.
- Ocular Pathology Laboratory: automated tissue processor and embedding center, crystat, and rotary microtome.
- Analytical Laboratory: sample processing facilities, HPLC, GCMS, and LCMSMS.
- Animal Surgery and Animal Rooms: surgical microscope, animal holding facilities.
- Microscopy Laboratory: fluorescence microcopy with imaging system, laser capture micro-dissection system.
We conduct research in the following fields with cross utilization of technologies on various disease entities:
- Molecular genetics
- Genomic studies
- Cell biology
- Stem cell biology
- Toxicology studies
- Herbal medicine & pharmacology
- Experimental animal models
- Epidemiology of eye diseases
- Cornea and external eye diseases
- Refractive errors
- High myopia
- Macular and retinal diseases
- Ocular oncology: retinoblastoma
- Pediatric ophthalmology
Recent research programme: Adipose-derived mesenchymal stem cell study
We will recruit systemic and local applications of autologous mesenchymal stem cells isolated from adipose tissue for various ocular disorders, including glaucoma, age-related macular degeneration, diabetic retinopathy, retinitis pigmentosa as well as uveitis. We will evaluate the efficacy and safety of treatment by visual acuity and visual field examination, color vision test, slip-lamp biomicroscopy, Goldmann applanation tonometry, dilated funduscopy, gonioscopy, fluorescence angiography, optical coherent tomography, electroretinography (ERG) and multifocal ERG.
Ongoing Major Basic Science Research Programmes:
- Genomic investigations, gene mapping and molecular genetics of glaucoma, age-related macular degeneration, and high myopiaGenome wide association studies with SNP and microsatellite marker scanning, family linkage analysis, fine mapping, sequencing, cloning, and gene function analysis to identify disease loci and characterize disease genes. More than 50 families, 2,000 patients and 500 control subjects are involved in these studies.
- Primary Open Angle Glaucoma (POAG)We were the first group to propose and confirm gain-of-function pathology in glaucoma caused by myocilin mutation. We have also identified 2 novel POAG chromosomal loci, GLCA1M and GLCA1N. We have recently identified a new susceptible gene for glaucoma and are currently characterizing its structure and properties. We participate in mapping CAV1 and CAV2 for POAG.
- Chen JH, Wang D, Huang C, Zheng Y, Chen H, Pang CP, Zhang M. Interactive effects of ATOH7 and RFTN1 in association with adult-onset primary open angle glaucoma. Invest Ophthalmol Vis Sci. 2012 Feb;53:779-85.
- Thorleifsson G, Walters GB, Hewitt AW, Masson G, Helgason A, DeWan A, Sigurdsson A, Jonasdottir A, Gudjonsson SA, Magnusson KP, Stefansson H, Lam DS, Tam PO, Gudmundsdottir GJ, Southgate L, Burdon KP, Gottfredsdottir MS, Aldred MA, Mitchell P, St Clair D, Collier DA, Tang N, Sveinsson O, Macgregor S, Martin NG, Cree AJ, Gibson J, Macleod A, Jacob A, Ennis S, Young TL, Chan JC, Karwatowski WS, Hammond CJ, Thordarson K, Zhang M, Wadelius C, Lotery AJ, Trembath RC, Pang CP, Hoh J, Craig JE, Kong A, Mackey DA, Jonasson F, Thorsteinsdottir U, Stefansson K. Common variants near CAV1 and CAV2 are associated with primary open-angle glaucoma. Nat Genet. 2010 Oct;42:906-9.
- Fan BJ, Liu K, Wang DY, Tham CC, Tam PO, Lam DS, Pang CP*. Association of polymorphisms of tumor necrosis factor and tumor protein p53 with primary open-angle glaucoma. Invest Ophthalmol Vis Sci. 2010 Aug;51:4110-6.
- Jia LY, Gong B, Pang CP, Huang Y, Lam DS, Wang N, Yam GH. Correction of the disease phenotype of myocilin-causing glaucoma by a natural osmolyte. Invest Ophthalmol Vis Sci. 2009 Aug;50:3743-9.
- Fan BJ, Wang DY, Tham CCY, Lam DSC, Pang CP. Gene expression profiles of human trabecular meshwork cells induced by triamcinolone and dexamethasone: putative genes for ocular hypertension. Invest Ophthalmol Vis Sci 2008; 49:1886-97.
- Wang DY, Fan BJ, Chua JKH, Tam POS, Leung CKS, Lam DSC, Pang CP. A genome-wide scan maps a novel juvenile-onset primary open angle glaucoma locus to chromosome 15q. Invest Ophthal Vis Sci 2006; Dec 47:5315-21.
- Pang CP, Fan BJ, Canlas O, Wang DY, Tam POS, Lam DSC, Fan DSP, Lam CY, Raymond V, Ritch R. A genome-wide scan maps a novel juvenile-onset primary open angle glaucoma locus to chromosome 5q. Mol Vision 2006 Feb;12:85-92.
- Pang CP, Leung YF, Fan BJ, Baum L, Tong WC, Lee WS, Chua JKH, Fan DSP, Lui Y, Lam DSC. TIGR/MYOC Gene sequence alterations in individuals with and without primary open angle glaucoma. Invest Ophthal Vis Sci 2002; 43:3231-5.
- Primary Angle Closure Glaucoma (PACG):
We participate in mapping the first loci for PACG.
- Vithana EN, Khor CC, Qiao C, Nongpiur ME, George R, Chen LJ, Do T, Abu-Amero K, Huang K, Low S, Tajudin LS, Perera SA, Cheng CY, Xu L, Jia H, Ho CL, Sim KS, Wu RY, Tham CC, Chew PT, Su DH, Oen FT, Sarangapani S, Soumittra N, Osman EA, Wong HT, Tang G, Fan S, Meng H, Huong DT, Wang H, Feng B, Baskaran M, Shantha B, Ramprasad VL, Kumaramanickavel G, Iyengar SK, How AC, Lee KY, Sivakumaran TA, Yong VH, Ting SM, Li Y, Wang YX, Tay WT, Sim X, Lavanya R, Cornes BK, Zheng YF, Wong TT, Loon SC, Yong VK, Waseem N, Yaakub A, Chia KS, Allingham RR, Hauser MA, Lam DS, Hibberd ML, Bhattacharya SS, Zhang M, Teo YY, Tan DT, Jonas JB, Tai ES, Saw SM, Hon DN, Al-Obeidan SA, Liu J, Chau TN, Simmons CP, Bei JX, Zeng YX, Foster PJ, Vijaya L, Wong TY, Pang CP, Wang N, Aung T. Genome-wide association analyses identify three new susceptibility loci for primary angle closure glaucoma. Nat Genet. 2012 Oct;44:1142-6.
- Age-related macular degeneration (AMD):
We identified a major AMD gene, HTRA1, in collaboration with a Yale University research group. We are currently investigating the mechanism of AMD through characterization of the biological functions of HTRA1 and other currently known AMD genes.
- Liu K, Chen LJ, Tam PO, Shi Y, Lai TY, Liu DT, Chiang SW, Yang M, Yang Z, Pang CP. Associations of the C2-CFB-RDBP-SKIV2L locus with age-related macular degeneration and polypoidal choroidal vasculopathy. Ophthalmology. 2013 Apr;120(4):837-43.
- Liang XY, Lai TY, Liu DT, Fan AH, Chen LJ, Tam PO, Chiang SW, Ng TK, Lam DS, Pang CP. Differentiation of exudative age-related macular degeneration and polypoidal choroidal vasculopathy in the ARMS2/HTRA1 locus. Invest Ophthalmol Vis Sci. 2012 May;53:3175-82
- Ng TK, Chen LJ, Liu DTL, Tam POS, Chan WM, Liu K, Hu YJ, Chong KKL, Lau CSL, Chiang SWY, Lam DSC, Pang CP. Multiple gene polymorphisms in the Complement Factor H gene are associated with exudative age-related macular degeneration in Chinese. Invest Ophthalmol Vis Sci 2008 Aug; 49:3312-7.
- Tam POS, Ng TK, Liu DTL, Chan WM, Chiang SWY, Chen LJ, DeWan A, Hoh J, Lam DSC, Pang CP. HTRA1 variants in exudative age-related macular degeneration and interactions with smoking and CFH. Invest Ophthalmol Vis Sci 2008 Jun;48:2357-65.
- DeWan A, Liu M, Hartman S, Zhang S, Liu DTL, Zhao C, Tam POS, Chan WM, Lam DSC, Snyder M, Barnstable C, Pang CP, Hoh J. HTRA serine protease predisposes Asians to age-related macular degeneration. Science Nov 2006;314:989-92.
- High myopia (HM):
We published the first candidate gene for high myopia, TGIF, which contributes modifying effects. We have identified a new HM locus, MYP16. We also discovered dinucleotide repeats in the PAX6 P1 promoter are associated with high myopia. Currently we are continuing our mapping for the myopia gene.
- Khor CC, Miyake M, Chen LJ, Shi Y, Barathi VA, Qiao F, Nakata I, Yamashiro K, Zhou X, Tam PO, Cheng CY, Tai ES, Vithana EN, Aung T, Teo YY, Wong TY, Moriyama M, Ohno-Matsui K, Mochizuki M, Matsuda F; Nagahama Study Group, Yong RY, Yap EP, Yang Z, Pang CP, Saw SM, Yoshimura N. Genome-wide association study identifies ZFHX1B as a susceptibility locus for severe myopia. Hum Mol Genet. 2013 Aug 19.[Epub ahead of print]
- Shi Y, Gong B, Chen L, Zuo X, Liu X, Tam PO, Zhou X, Zhao P, Lu F, Qu J, Sun L, Zhao F, Chen H, Zhang Y, Zhang D, Lin Y, Lin H, Ma S, Cheng J, Yang J, Huang L, Zhang M, Zhang X, Pang CP, Yang Z. A genome-wide meta-analysis identifies two novel loci associated with high myopia in the Han Chinese population. Hum Mol Genet. 2013 Jun;22(11):2325-33
- Ng TK, Lam CY, Lam DSC, Chiang SWY, Tam POS, Wang DY, Fan BJ, Yam GHF, Fan DSP, Pang CP. AC and AG dinucleotide repeats in the PAX6 P1 promoter are associated with high myopia. Mol Vision 2009 Nov;15:2239-48.
- Lam CY, Tam POS, Fan DSP, Fan BJ, Wang DY, Lee CWS, Pang CP, Lam DSC. A genome-wide scan maps a novel high myopia locus to 5p15. Invest Ophthalmol Vis Sci 2008 Sep; 49:3768-78.
- Lam DSC, Lee WS, Leung YF, Tam POS, Fan DSP, Fan BJ, Pang CP. TGFB-induced factor (TGIF) – a candidate gene for high myopia. Invest Ophthal Vis Sci 2003;44;1012-5.
- Molecular mechanisms of Bietti crystalline dystrophy, retinitis pigmentosa Graves’ ophthalmopathy, corneal dystrophies, congenital cataractsWe investigate the pathologic mechanisms of specific eye diseases with serious consequences of visual impairment and even blindness. Our results have been contributing to understanding of the pathogenesis for prevention and treatment.
- Bietti crystalline dystrophy (BCD):
Our genotyping, clinical phenotyping, and biochemical studies have discovered the association with dysfunctions of lipid metabolism.
- Lai T, Chu KO, Chan KP, Ng TK, Yam GH, Lam D, Pang CP. Alterations in serum fatty acid concentrations and desaturase activities in Bietti crystalline dystrophy unaffected by CYP4V2 genotypes. Invest Ophthalmol Vis Sci. 2009 Sep 24. [Epub ahead of print]
- Lai TY, Ng TK, Tam PO, Yam GH, Ngai JW, Chan WM, Liu DT, Lam DS, Pang CP. Genotype phenotype analysis of Bietti’s crystalline dystrophy in patients with CYP4V2 mutations. Invest Ophthalmol Vis Sci. 2007 Nov; 48:5212-20.
- Chan WM, Pang CP, Leung ATS, Fan DSP, Cheng ACK, Lam DSC. Bietti’s crystalline retinopathy in a family with all the three male siblings being affected. Arch Ophthalmol 2000; 118:129-31.
- Graves’ ophthalmopathy (GO):
We have shown the contribution of CTLA-4 to Graves Disease but not GO. Currently we are collecting data on the autoantibodies specific to GO and novel gene expressions for early diagnosis, and delineating genetic and environmental factors that lead to GO.
- Chong KKL, Chiang SWY, Wong GWK, Tam POS, Ng TK, Hu YJ, Yam GHF, Lam DSC, Pang CP. Association of CTLA-4 and IL-13 Gene polymorphisms with Graves’ disease and ophthalmopathy in Chinese children Invest Ophthalmol Vis Sci 2008; 49:2409-15.
- Retinitis pigmentosa (RP):
Our work has affirmed the contributions of the SNRNP200, NR2E3, RHO and RP1 genes on the mechanism of RP.
- Zhang X, Lai TY, Chiang SW, Tam PO, Liu DT, Chan CK, Pang CP, Zhao C, Chen LJ. Contribution of SNRNP200 sequence variations to retinitis pigmentosa. Eye 2013 Jul 26. [Epub ahead of print]
- Chen X, Zhao K, Sheng X, Li Y, Gao X, Zhang X, Kang X, Pan X, Liu Y, Jiang C, Shi H, Chen X, Rong W, Chen LJ, Lai TY, Liu Y, Wang X, Yuan S, Liu Q, Vollrath D, Pang CP, Zhao C. Targeted sequencing of 179 genes associated with hereditary retinal dystrophies and 10 candidate genes identifies novel and known mutations in patients with various retinal diseases. Invest Ophthalmol Vis Sci. 2013 Mar;54:2186-97.
- Abd El-Aziz MM, O’Driscoll CA, Kaye RS, Barragan I, El-Ashry MF, Borrego S, Antiñolo G, Pang CP, Webster AR, Bhattacharya SS. Identification of novel mutations in the ortholog of Drosophila eyes shut gene (EYS) causing autosomal recessive retinitis pigmentosa. Invest Ophthalmol Vis Sci. 2010 Aug;51:4266-72
- Yang YP, Zhang X, Chen LY, Chiang SWY, Tam POS, Lai TYY, Chan CKM, Wang N, Lam DSC, Pang CP. NR2E3 but not NRL mutations are associated with retinitis pigmentosa. Invest Ophthalmol Vis Sci 2009 Nov 20. [Epub ahead of print]
- Chen LY, Lai TYY, Tam POS, Chiang SWY, Zhang X, Lam S, Lai RYK, Lam DSC, Pang CP. Compound heterozygosity of two novel truncation mutations in RP1 causing autosomal recessive retinitis pigmentosa. Invest Ophthalmol Vis Sci 2009 Nov 20. [Epub ahead of print].
- Zhao C, Bellur DL, Lu S, Zhao F, Grassi MA, Bowne SJ, Sullivan LS, Daiger SP, Chen LJ, Pang CP, Zhao KX, Staley SP, Larsson C. Autosomal dominant retinitis pigmentosa caused by a mutation in SNRNP200, a gene required for unwinding of U4/U6 snRNAs. Amer J Hum Genet 2009 Oct 28. [Epub ahead of print].
- Corneal dystrophies:
In collaboration with Singapore Eye Research Institute, a novel gene in an endothelial form of cornea dystrophy has been identified
- Yam GH, Wang K, Jhanji V, Choy KW, Baum L, Pang CP. In vitro amyloid aggregate forming ability of TGFBI mutants that cause corneal dystrophies. Invest Ophthalmol Vis Sci. 2012 Aug;53(9):5890-8
- Vithana EN, Morgan PE, Ramprasad V, Tan DT, Yong VH, Venkataraman D, Venkatraman A, Yam GH, Nagasamy S, Law RW, Rajagopal R, Pang CP, Kumaramanickevel G, Casey JR, Aung T. SLC4A11 mutations in Fuchs endothelial corneal dystrophy (FECD). Hum Mol Genet. 2008 Mar; 17:656-66.
- Mehta JS, Vithana EN, Tan DT, Yong VH, Yam GH, Law RW, Chong WG, Pang CP, Aung T. Analysis of the posterior polymorphous corneal dystrophy 3 gene, TCF8, in late-onset Fuchs endothelial corneal dystrophy. Invest Ophthalmol Vis Sci. 2008 Jan;49:184-8.
We study the molecular mechanisms of anterior uveitis. Specific genotyping and interactive analysis have shown complex involvement of susceptible genes. We have shown HLA-B27 status affecting interactive effects of MnSOD and CCL2 in anterior uveitis. Our further investigations give evidence of strong differential risks due to variants in CFH and CFB in the complement alternative pathway, while other complement pathway genes, SERPING1 (C1INH), C3 and C5, as well as IL2_21 region and IL27 involving in T-cell response, confer limited risk. There are ongoing genome wide study and target sequencing programmes that will lead to identification of major genes in specific uveitis forms
- Yang MM, Lai TY, Tam PO, Chiang SW, Ng TK, Rong SS, Pang CP. Association of CFH and SERPING1 polymorphisms with anterior uveitis. Br J Ophthalmol. 2013 Aug 21.[Epub ahead of print]
- Yang MM, Lai TY, Luk FO, Pang CP. The roles of genetic factors in uveitis and their clinical significance. Retina. 2013 Jul 30. [Epub ahead of print]
- Yang MM, Lai TY, Tam PO, Chiang SW, Ng TK, Liu K, Pang CP. Association of C2 and CFB polymorphisms with anterior uveitis. Invest Ophthalmol Vis Sci. 2012 Jul;53:4969-74
- Lan C, Tam PO, Chiang SW, Chan CK, Luk FO, Lee GK, Ngai JW, Law JS, Lam DS, Pang CP, Lai TY. Manganese superoxide dismutase and chemokine gene polymorphisms in Chinese patients with anterior uveitis. Invest Ophthalmol Vis Sci. 2009 Dec;50:5596-60.
- Congenital cataracts:
We use an exclusion strategy to identify the disease causing mutations of specific congenital families. Characterizing the gene functions and properties throws light on the mechanism of cataract development.
- Gong B, Zhang LY, Lam DS, Pang CP, Yam GH. Sodium 4-phenylbutyrate ameliorates the effects of cataract-causing mutant gammaD-crystallin in cultured cells. Mol Vision. 2010 Jun;16:997-1003
- Zhang LY, Gong B, Tong JP, Fan DS, Chiang SW, Lou D, Lam DS, Yam GH, Pang CP. A novel gammaD-crystallin mutation causes mild changes in protein properties but leads to congenital coralliform cataract. Mol Vis. 2009 Aug;15:1521-9.
- Lou D, Tong JP, Zhang LY, Chiang SW, Lam DS, Pang CP. A novel mutation in CRYBB2 responsible for inherited coronary cataract. Eye 2009 May;23:1213-20.
- Zhang LY, Yam GH, Tam PO, Lai RY, Lam DS, Pang CP, Fan DS. An alphaA-crystallin gene mutation, Arg12Cys, causing inherited cataract-microcornea exhibits an altered heat-shock response. Mol Vis. 2009 Jun;15:1127-38.
- Zhang LY, Yam GHF, Fan DSP, Tam POS, Lam DSC, Pang CP. A novel deletion variant of gamma D-crystallin responsible for congenital nuclear cataract. Mol Vision 2007 Nov;13:2096-104.
- Molecular characterization
and epi-genetics of retinoblastoma (Rb).
We are the first group to delineate effects of loss of heterozygosity, microsattelite instability, promoter hypermethylation of tumor suppressor genes, RB1, MLH1, RASSlFA, and DNA repair gene (MGMT) on the tumorigenesis of retinoblastoma. Currently we are characterizing Rb genomics and chromosomal copy number variation.
- Ren R, Liu W, Huang L, Liu DT, Choy KW, Shi J, Zhao J, Zhao B, Guan M, Shields CL, Pang CP, Li B, Yam GH. Role of B lymphoma Mo-MLV insertion region 1 in the oncogenic behavior of retinoblastomas. Mol Vis. 2013;19:561-74
- Lau CS, Yu CB, Wong HK, Fan D, Mak HT, Wong KW, Lam DS, Pang CP, Choy KW. Allelic imbalance at 13q31 is associated with reduced GPC6 in Chinese with sporadic retinoblastoma. Br J Ophthalmol. 2009 Sep 1. [Epub ahead of print]
- Choy KW, Wang CC, Ogura A, Lau TK, Rogers MS, Ikeo K, Gojobori T, Lam DS, Pang CP. Genomic annotation of 15,809 ESTs identified from pooled early gestation human eyes. Physiol Genomics. 2006 Mar; 25:9-15.
- Choy KW, Lee TC, Cheung KF, Fan DSP, Lo KW, Beaverson KL, Abramson DH, Lam DSC, Yu CBO, Pang CP, Clinical implications of promoter hypermethylation in RASSF1A and MGMT in retinoblastoma. Neoplasia 2005 Mar; 7:200-6.
- Choy KW, Pang CP, Fan DSP, Lee TC, Wang JH, Abramson DH, Lo KW, To KF, Yu CBO, Beaverson KL, Cheung KF, Lam DSC. Microsatellite instability and MLH1 promoter methylation in human retinoblastoma. Invest Ophthal Vis Sci 2004; 45:3404-9.
- Choy KW, Pang CP, To KF, Yu CBO, Ng JSK, Lam DSC. Impaired expression and promoter hypermethylation of O6-methylguanine-DNA methytransferase (MGMT) frequently occur in retinoblastoma tissues. Invest Ophthal Vis Sci 2002; 43:1344-9.
- Molecular biology and toxicity studies of ocular cells: trabecular meshwork cells, retinal ganglion cells, retinal pigment epithelial cells, human endothelial vascular cells. Biological effects of herbal medicine.
We have been conducting series of cellular and molecular studies to examine toxicity and biological effects of new drug regimens and herbal medicine.
- Cen LP, Luo JM, Zhang CW, Fan YM, Song Y, So KF, van Rooijen N, Pang CP, Lam DSC, Cui Q. Chemotactic effect of ciliary neurotrophic factor on macrophages in retinal ganglion cell survival and axonal regeneration. Invest Ophthalmol Vis Sci 2007 Sep;48:4257-66.
- Tong JP, Lam DSC, Chan WM, Choy KW, Chan KP, Pang CP. Effects of triamcinolone on the expression of VEGF and PEDF in human retinal pigment epithelium (ARPE19) and human umbilical vein endothelial (HUVE) cells. Mol Vision 2006 December;12:1490-5.
- Yeung CK, Chan KP, Chiang WY, Pang CP, Lam DSC. The toxic and stress responses of cultured human retinal pigmented epithelium (ARPE19) and human glial (SVG) cells in the presence of triamcinolone. Invest Ophthal Vis Sci 2003; 44:5293-300.
- Yam HF, Kwok AKH, Chan KP, Lai TYY, Chu KY, Lam DSC, Pang CP. Effect of indocyanine green and illumination on gene expression in human retinal pigment epithelial cells. Invest Ophthal Vis Sci 2003;44:370-7.
- Molecular biology of ocular stem cells.
We published the first paper on miRNA characterization in human corneal progenitor cells. We study the biology of cornea progenitor cells, conventionally termed limbal stem cells, with a view to identify specific surface markers and understand the regulatory mechanisms of their differentiations.
- Huang L, Liang J, Geng Y, Tsang WM, Yao X, Jhanji V, Zhang M, Cheung HS, Pang CP, Yam GH. Directing adult human periodontal ligament-derived stem cells to retinal fate. Invest Ophthalmol Vis Sci. 2013 Jun;54(6):3965-74.
- Ng TK, Carballosa CM, Pelaez D, Wong HK, Choy KW, Pang CP, Cheung HS. Nicotine alters microRNA expression and hinders human adult stem cell regenerative potential. Stem Cells Dev. 2013 Mar;22(5):781-90.
- Lee SK, Teng Y, Wong HK, Ng TK, Huang L, Lei P, Choy KW, Liu Y, Zhang M, Lam DS, Yam GH, Pang CP. MicroRNA-145 Regulates Human Corneal Epithelial Differentiation. PLoS One. 2011;6(6):e21249. Epub 2011 Jun 20.
- Yam HF, Lam DSC, Pang CP. Changes of nuclear matrix in long term culture of limbal epithelial cells. Cornea 2002; 21:215-9.
- Yam GHF, Pang CP, Fan DSP, Fan B-J, Yu EYW, Lam DSC. Growth factor changes in ex vivo expansion of human limbal epithelial cells on human amniotic membrane. Cornea 2002;21:101-5.
- Herbal molecules for treatment of eye diseases
Herbal medicine has been in use for treatment of human ailments for millenniums across civilizations. Traditional herbal medicine is still in use today in therapies in all developed regions. Almost all such therapies involve mixed formulae with unspecified extraction procedures of a wide spectrum of plants. For eye diseases, our search of the literature in English has resulted in 7 categories of plants or herbal products described for ocular effects: carotenoids, Ginkgo biloba extract, androcyanins, resevratrol, catechins,omega-3-fatty acids, and isoliquiritigenins.We had shown that Ginkgo biloba extract (GBE) effectively suppressed steroid-induced ocular hypertension in rabbits, possibly mediated by preventing steroid-associated myocilin expression in trabecular meshwork cells, which became less prone to apoptosis. GBE, therefore, can preserve the functional outflow pathway and can be an alternative therapeutic agent for steroid-induced ocular hypertension. Among isoliquiritigenin (ISL) from licorice, epigallocatechin gallate (EGCG) from green tea, resveratrol (Rst) from grapes, and gambogic acid from resin of Garcinia hurburyi, we found ISL, EGCG, and Rst highly effective in suppressing endothelial cell proliferation and migration, with low cytotoxicity. They are potentially useful for anti-angiogenic therapies by virtue of their low effective dosages and small molecular sizes for easy penetration through tissue cells. We have adopted in vivo models to study these molecules in regulated angiogenesis using chick chorioallantoic membrane vascularization and pathological angiogenesis using chemical-induced corneal neovascularization, laser photocoagulation-induced choroidal neovascularization and oxygen-induced retinal neovascularization in mice. Our experimental rat models include sodium iodate induced retinal degeneration and LPS induced ocular inflammation.
- Jhanji V, Liu H, Law K, Lee VY, Huang SF, Pang CP, Yam GH. Isoliquiritigenin from licorice root suppressed neovascularisation in experimental ocular angiogenesis models. Br J Ophthalmol. 2011 Sep;95:1309-15.
- Chu KO, Chan KP, Wang CC, Chu CY, Li WY, Choy KW, Rogers MS, Pang CP. Green tea catechins and their oxidative protection in the rat eye. J Agric Food Chem. 2010 Feb 10;58:1523-34
- Jia L-Y, Sun L, Fan DSP, Lam DSC, Pang CP, Yam GHF. Effect of topical Ginkgo biloba extract on steroid-induced changes in the trabecular meshwork and intraocular pressure. Arch Ophthalmol 2008 Dec;126:1700-6
- Wang CC, Chu KO, Chong WS, Li WY, Pang CP, Shum ASW, Lau TK and Rogers MS. Tea epigallocatechin-3-gallate increased 8-isoprostane level and induced caudal regression in developing rat embryos. Free Radic Biol Med 2007 Aug; 43:519-527
- Advancements in analytical technologies
We do research especially on separation technologies to aid in our research on pharmacokinetics, drugs effects and nucleic acids studies. We are also utilizing proteomic technologies to analyze human tear in inflammatory eye diseases.
- Chu KO, Liu DT, Chan KP, Yang YP, Yam GH, Rogers MS, Pang CP. Quantification and structure elucidation of in vivo bevacizumab modification in rabbit vitreous humor after intravitreal injection. Mol Pharm. 2012 Nov 6. [Epub ahead of print]
- Pong JC, Chu CY, Chu KO, Poon TC, Ngai SM, Pang CP, Wang CC. Identification of hemopexin in tear film. Anal Biochem. 2010 Sep;404:82-5.
- Chu CY, Poon CW, Pong CF, Pang CP, Wang CC. Human normal tear proteome. Graefes Arch Clin Exp Ophthalmol. 2009 Jun;247:725-7.
- Chu KO, Wang CC, Pang CP, Rogers MS. Method to determine stability and recovery of carboprost and misoprostol in infusion preparation. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 September; 857:83-91.